› 1umg

X-ray diffraction
1.8Å resolution

Crystal structure of fructose-1,6-bisphosphatase

Released:
DOI: 10.2210/pdb1umg/pdb
PDB entry 1umg coloured by chain, front view.
PDB entry 1umg coloured by chain, side view.
PDB entry 1umg coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea.
Nishimasu H, Fushinobu S, Shoun H, Wakagi T
Structure 12 949-59 (2004)
PMID: 15274916

Function and Biology Details

Reactions catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphate aldolase/phosphatase Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 40.1 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F9VMT6 (Residues: 2-363; Coverage: 94%)
Gene names: STK_03180, fbp
Sequence domains: Fructose-1,6-bisphosphatase

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand 2FP 1 x 2FP
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: I422
Unit cell:
a: 111.778Å b: 111.778Å c: 153.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.197
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
1 more

3 mentions without citation

Assembly reflects evolution of protein complexes.
Levy et al. (2008)
2 more