› 1umw

X-ray diffraction
1.9Å resolution

Structure of a human Plk1 Polo-box domain/phosphopeptide complex

Released:
DOI: 10.2210/pdb1umw/pdb
PDB entry 1umw coloured by chain, front view.
PDB entry 1umw coloured by chain, side view.
PDB entry 1umw coloured by chain, top view.
Source organisms:
Primary publication:
The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain.
Elia AE, Rellos P, Haire LF, Chao JW, Ivins FJ, Hoepker K, Mohammad D, Cantley LC, Smerdon SJ, Yaffe MB
Cell 115 83-95 (2003)
PMID: 14532005

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156754 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53350 (Residues: 367-603; Coverage: 39%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
PEPTIDE Chains: E, F
Molecule details ›
Chains: E, F
Length: 7 amino acids
Theoretical weight: 853 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P21
Unit cell:
a: 62.352Å b: 79.518Å c: 61.993Å
α: 90° β: 93.26° γ: 90°
R-values:
R R work R free
0.229 0.23 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

87 review citations

Polo-like kinases and the orchestration of cell division.
Barr et al. (2004)
86 more

16 mentions without citation

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
15 more