1un1

X-ray diffraction
2.1Å resolution

Xyloglucan endotransglycosylase native structure.

Released:

Function and Biology Details

Reaction catalysed:
Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-184600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Xyloglucan endotransglucosylase protein 34 Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.53 KDa
Source organism: Populus tremula
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8GZD5 (Residues: 23-294; Coverage: 100%)
Gene names: XET16A, XTH16-34
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P63
Unit cell:
a: 188.762Å b: 188.762Å c: 45.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.232
Expression system: Komagataella pastoris