› 1un6

X-ray diffraction
3.1Å resolution

THE CRYSTAL STRUCTURE OF A ZINC FINGER - RNA COMPLEX REVEALS TWO MODES OF MOLECULAR RECOGNITION

Released:
DOI: 10.2210/pdb1un6/pdb
PDB entry 1un6 coloured by chain, front view.
PDB entry 1un6 coloured by chain, side view.
PDB entry 1un6 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Crystal structure of a zinc-finger-RNA complex reveals two modes of molecular recognition.
Lu D, Searles MA, Klug A
Nature 426 96-100 (2003)
PMID: 14603324

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-136448 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription factor IIIA Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 87 amino acids
Theoretical weight: 10.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03001 (Residues: 126-212; Coverage: 24%)
Gene name: gtf3a
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5S ribosomal RNA Chains: E, F
Molecule details ›
Chains: E, F
Length: 61 nucleotides
Theoretical weight: 19.71 KDa
Source organism: Xenopus laevis

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 13 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A, SRS BEAMLINE PX14.2
Spacegroup: C2
Unit cell:
a: 58.598Å b: 191.593Å c: 79.77Å
α: 90° β: 101.51° γ: 90°
R-values:
R R work R free
0.216 0.216 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

32 review citations

Multiple modes of RNA recognition by zinc finger proteins.
Hall TM. (2005)
31 more

16 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
15 more