› 1upu

X-ray diffraction
2.5Å resolution

STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE, MUTANT C128V, BOUND TO PRODUCT URIDINE-1-MONOPHOSPHATE (UMP)

Released:
DOI: 10.2210/pdb1upu/pdb
PDB entry 1upu coloured by chain, front view.
PDB entry 1upu coloured by chain, side view.
PDB entry 1upu coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding.
Schumacher MA, Carter D, Scott DM, Roos DS, Ullman B, Brennan RG
EMBO J 17 3219-32 (1998)
PMID: 9628859

Function and Biology Details

Reaction catalysed: 
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.54 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q26998 (Residues: 21-244; Coverage: 92%)
Gene name: uprt
Sequence domains: Uracil phosphoribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand U5P 4 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 60.28Å b: 141.77Å c: 71.42Å
α: 90° β: 114.93° γ: 90°
R-values:
R R work R free
0.148 0.148 not available
Expression system: Escherichia coli

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Citations

8 review citations

Structural analyses reveal two distinct families of nucleoside phosphorylases.
Pugmire et al. (2002)
7 more

1 mention without citation

Complex fitness landscape shapes variation in a hyperpolymorphic species.
Stolyarova et al. (2022)