› 1ush

X-ray diffraction
1.73Å resolution

5'-NUCLEOTIDASE FROM E. COLI

Released:
DOI: 10.2210/pdb1ush/pdb
PDB entry 1ush coloured by chain, front view.
PDB entry 1ush coloured by chain, side view.
PDB entry 1ush coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site.
Knöfel T, Sträter N
Nat Struct Biol 6 448-53 (1999)
PMID: 10331872

Function and Biology Details

Reactions catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
UDP-sugar + H(2)O = UMP + alpha-D-aldose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein UshA Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P07024 (Residues: 1-550; Coverage: 100%)
Gene names: JW0469, b0480, ushA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CO3 1 x CO3
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P41212
Unit cell:
a: 83.61Å b: 83.61Å c: 181.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.219
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Calcineurin: form and function.
Rusnak et al. (2000)
4 more

16 mentions without citation

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
15 more