› 1uvi

X-ray diffraction
2.15Å resolution

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 with 6nt RNA

Released:
DOI: 10.2210/pdb1uvi/pdb
PDB entry 1uvi coloured by chain, front view.
PDB entry 1uvi coloured by chain, side view.
PDB entry 1uvi coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.
Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM
Structure 12 307-16 (2004)
PMID: 14962391

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145662 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
5'-R(*UP*UP*UP*UP*CP*CP)-3' Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21
Unit cell:
a: 105.13Å b: 93.711Å c: 140.741Å
α: 90° β: 101.22° γ: 90°
R-values:
R R work R free
0.23 0.23 0.256
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Structure-function relationships among RNA-dependent RNA polymerases.
Ng et al. (2008)
6 more

6 mentions without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)
5 more