› 1uwh

X-ray diffraction
2.95Å resolution

The complex of wild type B-RAF and BAY439006.

Released:
DOI: 10.2210/pdb1uwh/pdb
PDB entry 1uwh coloured by chain, front view.
PDB entry 1uwh coloured by chain, side view.
PDB entry 1uwh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of activation of the RAF-ERK signaling pathway by oncogenic mutations of B-RAF.
Wan PT, Garnett MJ, Roe SM, Lee S, Niculescu-Duvaz D, Good VM, Jones CM, Marshall CJ, Springer CJ, Barford D, Marais R
Cell 116 855-67 (2004)
PMID: 15035987

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15056 (Residues: 448-723; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BAX 2 x BAX
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P41212
Unit cell:
a: 110.28Å b: 110.28Å c: 143.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

494 review citations

Cancer genes and the pathways they control.
Vogelstein et al. (2004)
493 more

79 mentions without citation

Dynamics-Driven Allostery in Protein Kinases.
Kornev et al. (2015)
78 more