› 1uwp

X-ray diffraction
1.2Å resolution

Initial Events in the Photocycle of Photoactive Yellow Protein

Released:
DOI: 10.2210/pdb1uwp/pdb
PDB entry 1uwp coloured by chain, front view.
PDB entry 1uwp coloured by chain, side view.
PDB entry 1uwp coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Initial events in the photocycle of photoactive yellow protein.
Kort R, Hellingwerf KJ, Ravelli RB
J Biol Chem 279 26417-24 (2004)
PMID: 15026418

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: X
Molecule details ›
Chain: X
Length: 125 amino acids
Theoretical weight: 14.12 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand HC4 1 x HC4
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P63
Unit cell:
a: 65.922Å b: 65.922Å c: 40.584Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.137 0.136 0.157
Expression system: Escherichia coli

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Citations

8 review citations

When X-rays modify the protein structure: radiation damage at work.
Carugo et al. (2005)
7 more

1 mention without citation

Contradictions in X-ray structures of intermediates in the photocycle of photoactive yellow protein.
Kaila et al. (2014)