› 1uz4

X-ray diffraction
1.71Å resolution

Common inhibition of beta-glucosidase and beta-mannosidase by isofagomine lactam reflects different conformational intineraries for glucoside and mannoside hydrolysis

Released:
DOI: 10.2210/pdb1uz4/pdb
PDB entry 1uz4 coloured by chain, front view.
PDB entry 1uz4 coloured by chain, side view.
PDB entry 1uz4 coloured by chain, top view.
Source organism: Cellvibrio mixtus
Primary publication:
Common inhibition of both beta-glucosidases and beta-mannosidases by isofagomine lactam reflects different conformational itineraries for pyranoside hydrolysis.
Vincent F, Gloster TM, Macdonald J, Morland C, Stick RV, Dias FM, Prates JA, Fontes CM, Gilbert HJ, Davies GJ
Chembiochem 5 1596-9 (2004)
PMID: 15515081

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Man5A Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 50.23 KDa
Source organism: Cellvibrio mixtus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6QT42 (Residues: 26-456; Coverage: 95%)
Gene name: man5A
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand IFL 1 x IFL
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 91.625Å b: 102.37Å c: 50.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.165
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

β-Glucosidases.
Ketudat Cairns et al. (2010)
3 more

4 mentions without citation

Substrate recognition and hydrolysis by a family 50 exo-β-agarase, Aga50D, from the marine bacterium Saccharophagus degradans.
Pluvinage et al. (2013)
3 more