› 1v0f

X-ray diffraction
2.55Å resolution

Endosialidase of Bacteriophage K1F in complex with oligomeric alpha-2,8-sialic acid

Released:
DOI: 10.2210/pdb1v0f/pdb
PDB entry 1v0f coloured by chain, front view.
PDB entry 1v0f coloured by chain, side view.
PDB entry 1v0f coloured by chain, top view.
Source organism: Escherichia phage K1F
Primary publication:
Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F.
Stummeyer K, Dickmanns A, Mühlenhoff M, Gerardy-Schahn R, Ficner R
Nat Struct Mol Biol 12 90-6 (2005)
PMID: 15608653

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mature tail spike protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 666 amino acids
Theoretical weight: 74.13 KDa
Source organism: Escherichia phage K1F
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04830 (Residues: 245-910; Coverage: 63%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SIA
Carbohydrate polymer
2 bound ligands:
Ligand SLB 6 x SLB
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P2221
Unit cell:
a: 99.54Å b: 131.4Å c: 346.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.232
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration.
Schnaar et al. (2014)
25 more

3 mentions without citation

Unraveling virus relationships by structure-based phylogenetic classification.
Ng et al. (2020)
2 more