› 1v13

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE MUTANT HIS103ALA OF THE COLICIN E9 DNASE DOMAIN IN COMPLEX WITH ZN+2 (2.0 ANGSTROMS)

Released:
DOI: 10.2210/pdb1v13/pdb
PDB entry 1v13 coloured by chain, front view.
PDB entry 1v13 coloured by chain, side view.
PDB entry 1v13 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-based analysis of the metal-dependent mechanism of H-N-H endonucleases.
Maté MJ, Kleanthous C
J Biol Chem 279 34763-9 (2004)
PMID: 15190054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 52.693Å b: 61.441Å c: 91.107Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.23 0.307
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Colicin biology.
Cascales et al. (2007)
3 more

1 mention without citation

Trans-splicing facilitated by RNA pairing greatly expands sDscam isoform diversity but not homophilic binding specificity.
Hou et al. (2022)