› 1v15

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A)

Released:
DOI: 10.2210/pdb1v15/pdb
PDB entry 1v15 coloured by chain, front view.
PDB entry 1v15 coloured by chain, side view.
PDB entry 1v15 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-based analysis of the metal-dependent mechanism of H-N-H endonucleases.
Maté MJ, Kleanthous C
J Biol Chem 279 34763-9 (2004)
PMID: 15190054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114713 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Colicin-E9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 15.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain
5'-D(*GP*CP*GP*AP*TP*CP*GP*CP)-3' Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 93.145Å b: 123.31Å c: 110.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.242 0.329
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Colicin biology.
Cascales et al. (2007)
3 more

2 mentions without citation

Local conformational changes in the DNA interfaces of proteins.
Sunami et al. (2013)
1 more