› 1v1q

X-ray diffraction
2.1Å resolution

Crystal structure of PriB- a primosomal DNA replication protein of Escherichia coli

Released:
DOI: 10.2210/pdb1v1q/pdb
PDB entry 1v1q coloured by chain, front view.
PDB entry 1v1q coloured by chain, side view.
PDB entry 1v1q coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli.
Liu JH, Chang TW, Huang CY, Chen SU, Wu HN, Chang MC, Hsiao CD
J Biol Chem 279 50465-71 (2004)
PMID: 15383524

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Primosomal replication protein N Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 14.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07013 (Residues: 1-104; Coverage: 100%)
Gene names: JW4159, b4201, priB
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand CYS 3 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P212121
Unit cell:
a: 39.322Å b: 57.358Å c: 97.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
1 more

8 mentions without citation

A hand-off mechanism for primosome assembly in replication restart.
Lopper et al. (2007)
7 more