› 1v3e

X-ray diffraction
1.89Å resolution

Structure of the hemagglutinin-neuraminidase from human parainfluenza virus type III: complex with ZANAMAVIR

Released:
DOI: 10.2210/pdb1v3e/pdb
PDB entry 1v3e coloured by chain, front view.
PDB entry 1v3e coloured by chain, side view.
PDB entry 1v3e coloured by chain, top view.
Source organism: Human respirovirus 3
Primary publication:
Structure of the haemagglutinin-neuraminidase from human parainfluenza virus type III.
Lawrence MC, Borg NA, Streltsov VA, Pilling PA, Epa VC, Varghese JN, McKimm-Breschkin JL, Colman PM
J Mol Biol 335 1343-57 (2004)
PMID: 14729348

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hemagglutinin-neuraminidase glycoprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 48.11 KDa
Source organism: Human respirovirus 3
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6WJ03 (Residues: 142-572; Coverage: 75%)
Gene name: HN
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 2 x CA
Ligand SO4 2 x SO4
Ligand ZMR 2 x ZMR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B, RIGAKU
Spacegroup: P6222
Unit cell:
a: 218.451Å b: 218.451Å c: 109.904Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.219
Expression system: Trichoplusia ni

Quick links

Citations

37 review citations

Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme.
White et al. (2008)
36 more

7 mentions without citation

A second receptor binding site on human parainfluenza virus type 3 hemagglutinin-neuraminidase contributes to activation of the fusion mechanism.
Porotto et al. (2007)
6 more