› 1v8p

X-ray diffraction
2.52Å resolution

Crystal structure of PAE2754 from Pyrobaculum aerophilum

Released:
DOI: 10.2210/pdb1v8p/pdb
PDB entry 1v8p coloured by chain, front view.
PDB entry 1v8p coloured by chain, side view.
PDB entry 1v8p coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
Distant structural homology leads to the functional characterization of an archaeal PIN domain as an exonuclease.
Arcus VL, Bäckbro K, Roos A, Daniel EL, Baker EN
J Biol Chem 279 16471-8 (2004)
PMID: 14734548

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exonuclease VapC9 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 158 amino acids
Theoretical weight: 18.01 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZUJ3 (Residues: 1-133; Coverage: 100%)
Gene names: PAE2754, vapC9
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

1 bound ligand:
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 60.56Å b: 165.19Å c: 203.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.305
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
18 more

5 mentions without citation

Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis.
Miallau et al. (2009)
4 more