› 1v8z

X-ray diffraction
2.21Å resolution

X-ray crystal structure of the Tryptophan Synthase b2 Subunit from Hyperthermophile, Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1v8z/pdb
PDB entry 1v8z coloured by chain, front view.
PDB entry 1v8z coloured by chain, side view.
PDB entry 1v8z coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
The crystal structure of the tryptophan synthase beta subunit from the hyperthermophile Pyrococcus furiosus. Investigation of stabilization factors.
Hioki Y, Ogasahara K, Lee SJ, Ma J, Ishida M, Yamagata Y, Matsuura Y, Ota M, Ikeguchi M, Kuramitsu S, Yutani K
Eur J Biochem 271 2624-35 (2004)
PMID: 15206928

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 42.59 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U093 (Residues: 1-388; Coverage: 100%)
Gene names: PF1706, trpB1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 84.835Å b: 110.53Å c: 159.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.263
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

CATALASE2 Coordinates SA-Mediated Repression of Both Auxin Accumulation and JA Biosynthesis in Plant Defenses.
Yuan et al. (2017)
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4 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
3 more