› 1vb6

X-ray diffraction
1.56Å resolution

Crystal Structure of the heme PAS sensor domain of Ec DOS (oxygen-bound form)

Released:
DOI: 10.2210/pdb1vb6/pdb
PDB entry 1vb6 coloured by chain, front view.
PDB entry 1vb6 coloured by chain, side view.
PDB entry 1vb6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Kurokawa H, Watanabe M, Sagami I, Mikami B, Shimizu T

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen sensor protein DosP Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76129 (Residues: 1-139; Coverage: 17%)
Gene names: JW1484, b1489, dos, dosP, pdeO, yddU
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 47.894Å b: 69.337Å c: 81.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.232
Expression system: Escherichia coli BL21(DE3)

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