› 1vbl

X-ray diffraction
1.91Å resolution

Structure of the thermostable pectate lyase PL 47

Released:
DOI: 10.2210/pdb1vbl/pdb
PDB entry 1vbl coloured by chain, front view.
PDB entry 1vbl coloured by chain, side view.
PDB entry 1vbl coloured by chain, top view.
Source organism: Bacillus sp. TS-47
Entry authors: Nakaniwa T, Tada T, Yamaguchi A, Kitatani T, Takao M, Sakai T, Nishimura K

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectate lyase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 47.32 KDa
Source organism: Bacillus sp. TS-47
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q9AJM4 (Residues: 26-441; Coverage: 100%)
Gene name: pl47
Sequence domains: Pectate lyase
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3121
Unit cell:
a: 58.61Å b: 58.61Å c: 229.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.209
Expression system: Bacillus subtilis

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