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Solution NMR

Solution Structure of RSGI RUH-011, a UBA Domain from Arabidopsis cDNA

Released:
DOI: 10.2210/pdb1vek/pdb
PDB entry 1vek coloured by chain, ensemble of 20 models, front view.
PDB entry 1vek coloured by chain, ensemble of 20 models, side view.
PDB entry 1vek coloured by chain, ensemble of 20 models, top view.
Source organism: Arabidopsis thaliana
Entry authors: Higuchi Y, Abe T, Hirota H, Saito K, Koshiba S, Kigawa T, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 14 Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 8.63 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q8L6Y1 (Residues: 594-664; Coverage: 9%)
Gene names: At3g20630, F3H11_1, K10D20.17, K10D20.26, TTN6, UBP14
Sequence domains: UBA/TS-N domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Not provided

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