› 1vfh

X-ray diffraction
2Å resolution

Crystal structure of alanine racemase from D-cycloserine producing Streptomyces lavendulae

Released:
DOI: 10.2210/pdb1vfh/pdb
PDB entry 1vfh coloured by chain, front view.
PDB entry 1vfh coloured by chain, side view.
PDB entry 1vfh coloured by chain, top view.
Source organism: Streptomyces lavendulae
Primary publication:
Structural evidence that alanine racemase from a D-cycloserine-producing microorganism exhibits resistance to its own product.
Noda M, Matoba Y, Kumagai T, Sugiyama M
J Biol Chem 279 46153-61 (2004)
PMID: 15302886

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 41.05 KDa
Source organism: Streptomyces lavendulae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65YW7 (Residues: 1-378; Coverage: 100%)
Gene name: alr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 83.98Å b: 63.38Å c: 85.95Å
α: 90° β: 120.14° γ: 90°
R-values:
R R work R free
0.21 0.197 0.252
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure, function and dynamics in the mur family of bacterial cell wall ligases.
Smith CA. (2006)
4 more

5 mentions without citation

Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames).
Couñago et al. (2009)
4 more