› 1vj5

X-ray diffraction
2.35Å resolution

Human soluble Epoxide Hydrolase- N-cyclohexyl-N'-(4-iodophenyl)urea complex

Released:
DOI: 10.2210/pdb1vj5/pdb
PDB entry 1vj5 coloured by chain, front view.
PDB entry 1vj5 coloured by chain, side view.
PDB entry 1vj5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis.
Gomez GA, Morisseau C, Hammock BD, Christianson DW
Biochemistry 43 4716-23 (2004)
PMID: 15096040

Function and Biology Details

Reactions catalysed: 
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional epoxide hydrolase 2 Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 62.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34913 (Residues: 1-555; Coverage: 100%)
Gene name: EPHX2
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand P6G 1 x P6G
Ligand CIU 1 x CIU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P6522
Unit cell:
a: 93.51Å b: 93.51Å c: 245.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.266
Expression system: Spodoptera frugiperda

Quick links

Citations

24 review citations

Action of epoxyeicosatrienoic acids on cellular function.
Spector et al. (2007)
23 more

14 mentions without citation

SCOPe: Structural Classification of Proteins--extended, integrating SCOP and ASTRAL data and classification of new structures.
Fox et al. (2014)
13 more