› 1vtn

X-ray diffraction
2.5Å resolution

CO-CRYSTAL STRUCTURE OF THE HNF-3/FORK HEAD DNA-RECOGNITION MOTIF RESEMBLES HISTONE H5

Released:
DOI: 10.2210/pdb1vtn/pdb
PDB entry 1vtn coloured by chain, front view.
PDB entry 1vtn coloured by chain, side view.
PDB entry 1vtn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5.
Clark KL, Halay ED, Lai E, Burley SK
Nature 364 412-20 (1993)
PMID: 8332212

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114746 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 3-gamma Chain: C
Molecule details ›
Chain: C
Length: 102 amino acids
Theoretical weight: 12.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P55318 (Residues: 115-215; Coverage: 29%)
Gene names: FOXA3, HNF3G, TCF3G
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*CP*TP*AP*AP*GP*TP*CP*AP*AP*CP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*TP*GP*AP*CP*TP*TP*AP*GP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P31
Unit cell:
a: 74.6Å b: 74.6Å c: 85.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 not available
Expression system: Not provided

Quick links

Citations

205 review citations

Pioneer transcription factors: establishing competence for gene expression.
Zaret et al. (2011)
204 more

24 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
23 more