› 1vz8

X-ray diffraction
2.75Å resolution

Ornithine Acetyltransferase (ORF6 Gene Product - Clavulanic Acid Biosynthesis) from Streptomyces clavuligerus (SeMet structure)

Released:
DOI: 10.2210/pdb1vz8/pdb
PDB entry 1vz8 coloured by chain, front view.
PDB entry 1vz8 coloured by chain, side view.
PDB entry 1vz8 coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
X-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster.
Elkins JM, Kershaw NJ, Schofield CJ
Biochem J 385 565-73 (2005)
PMID: 15352873

Function and Biology Details

Reaction catalysed: 
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate N-acetyltransferase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 42.03 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DJQ5 (Residues: 1-393; Coverage: 100%)
Gene names: dclD, oat2
Sequence domains: ArgJ family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 60.792Å b: 180.194Å c: 73.326Å
α: 90° β: 90.08° γ: 90°
R-values:
R R work R free
0.235 0.235 0.288
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

The enzymes of β-lactam biosynthesis.
Hamed et al. (2013)
7 more

2 mentions without citation

Prediction of protein binding sites in protein structures using hidden Markov support vector machine.
Liu et al. (2009)
1 more