› 1w25

X-ray diffraction
2.7Å resolution

Response regulator PleD in complex with c-diGMP

Released:
DOI: 10.2210/pdb1w25/pdb
PDB entry 1w25 coloured by chain, front view.
PDB entry 1w25 coloured by chain, side view.
PDB entry 1w25 coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Structural basis of activity and allosteric control of diguanylate cyclase.
Chan C, Paul R, Samoray D, Amiot NC, Giese B, Jenal U, Schirmer T
Proc. Natl. Acad. Sci. U.S.A. 101 17084-9 (2004)
PMID: 15569936

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator PleD Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 50.39 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GZM2 (Residues: 2-454; Coverage: 100%)
Gene names: CCNA_02546, pleD
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 2 x MG
Ligand C2E 5 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P42212
Unit cell:
a: 135.87Å b: 135.87Å c: 169.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

45 review citations

Principles of c-di-GMP signalling in bacteria.
Hengge R. (2009)
44 more

28 mentions without citation

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
27 more