› 1w3d

Solution NMR

NMR structure of the peripheral-subunit binding domain of Bacillus stearothermophilus E2p

Released:
DOI: 10.2210/pdb1w3d/pdb
PDB entry 1w3d coloured by chain, ensemble of 20 models, front view.
PDB entry 1w3d coloured by chain, ensemble of 20 models, side view.
PDB entry 1w3d coloured by chain, ensemble of 20 models, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy.
Allen MD, Broadhurst RW, Solomon RG, Perham RN
FEBS J 272 259-68 (2005)
PMID: 15634348

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex Chain: A
Molecule details ›
Chain: A
Length: 55 amino acids
Theoretical weight: 5.81 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11961 (Residues: 118-171; Coverage: 13%)
Gene name: pdhC
Sequence domains: e3 binding domain
Structure domains: E3-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Escherichia coli BL21(DE3)

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Citations

16 citation in other articles

Ultra-fast barrier-limited folding in the peripheral subunit-binding domain family.
Ferguson et al. (2005)
15 more

5 mentions without citation

Nuclear magnetic resonance approaches in the study of 2-oxo acid dehydrogenase multienzyme complexes--a literature review.
Kumaran et al. (2013)
4 more