› 1w4r

X-ray diffraction
1.83Å resolution

Structure of a type II thymidine kinase with bound dTTP

Released:
DOI: 10.2210/pdb1w4r/pdb
PDB entry 1w4r coloured by chain, front view.
PDB entry 1w4r coloured by chain, side view.
PDB entry 1w4r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a type II thymidine kinase with bound dTTP.
Birringer MS, Claus MT, Folkers G, Kloer DP, Schulz GE, Scapozza L
FEBS Lett 579 1376-82 (2005)
PMID: 15733844

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase, cytosolic Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 195 amino acids
Theoretical weight: 21.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04183 (Residues: 15-194; Coverage: 77%)
Gene name: TK1
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TTP 8 x TTP
Ligand ZN 8 x ZN
Ligand DTU 1 x DTU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 157.496Å b: 122.92Å c: 115.274Å
α: 90° β: 130° γ: 90°
R-values:
R R work R free
0.16 0.159 0.189
Expression system: Escherichia coli

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Citations

2 review citations

Thymidine kinase 1 through the ages: a comprehensive review.
Bitter et al. (2020)
1 more

18 mentions without citation

N3-substituted thymidine bioconjugates for cancer therapy and imaging.
Khalil et al. (2013)
17 more