› 1w70

X-ray diffraction
1.46Å resolution

SH3 domain of p40phox complexed with C-terminal polyProline region of p47phox

Released:
DOI: 10.2210/pdb1w70/pdb
PDB entry 1w70 coloured by chain, front view.
PDB entry 1w70 coloured by chain, side view.
PDB entry 1w70 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains.
Massenet C, Chenavas S, Cohen-Addad C, Dagher MC, Brandolin G, Pebay-Peyroula E, Fieschi F
J Biol Chem 280 13752-61 (2005)
PMID: 15657040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146974 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neutrophil cytosol factor 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15080 (Residues: 174-228; Coverage: 16%)
Gene names: NCF4, SH3PXD4
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Neutrophil cytosol factor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P14598 (Residues: 360-372; Coverage: 3%)
Gene names: NCF1, NOXO2, SH3PXD1A
Sequence domains: NADPH oxidase subunit p47Phox, C terminal domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand TFA 2 x TFA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 39.64Å b: 50.49Å c: 68.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.
Bedard et al. (2007)
12 more

7 mentions without citation

Molecular basis for SH3 domain regulation of F-BAR-mediated membrane deformation.
Rao et al. (2010)
6 more