› 1w7s

X-ray diffraction
1.85Å resolution

Wild-Type Aequorea victoria Green Fluorescent Protein

Released:
DOI: 10.2210/pdb1w7s/pdb
PDB entry 1w7s coloured by chain, front view.
PDB entry 1w7s coloured by chain, side view.
PDB entry 1w7s coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Ultrafast and low barrier motions in the photoreactions of the green fluorescent protein.
van Thor JJ, Georgiev GY, Towrie M, Sage JT
J Biol Chem 280 33652-9 (2005)
PMID: 16033764

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.93 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli M15
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYS 4 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2
Unit cell:
a: 154.968Å b: 52.73Å c: 141.628Å
α: 90° β: 120.01° γ: 90°
R-values:
R R work R free
0.178 0.176 0.216
Expression system: Escherichia coli M15

Quick links

Citations

4 review citations

Excited state reactions in fluorescent proteins.
Meech SR. (2009)
3 more

17 mentions without citation

The fluorescent protein palette: tools for cellular imaging.
Day et al. (2009)
16 more