› 1w9t

X-ray diffraction
1.62Å resolution

Structure of a beta-1,3-glucan binding CBM6 from Bacillus halodurans in complex with xylobiose

Released:
DOI: 10.2210/pdb1w9t/pdb
PDB entry 1w9t coloured by chain, front view.
PDB entry 1w9t coloured by chain, side view.
PDB entry 1w9t coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
Family 6 carbohydrate binding modules recognize the non-reducing end of beta-1,3-linked glucans by presenting a unique ligand binding surface.
van Bueren AL, Morland C, Gilbert HJ, Boraston AB
J Biol Chem 280 530-7 (2005)
PMID: 15501830

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucan endo-1,3-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.67 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KG76 (Residues: 790-925; Coverage: 14%)
Gene name: BH0236
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
3 bound ligands:
Ligand NA 6 x NA
Ligand XYS 2 x XYS
Ligand XYP 4 x XYP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 30.711Å b: 40.967Å c: 56.039Å
α: 108.89° β: 105.87° γ: 90.03°
R-values:
R R work R free
0.121 0.118 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
7 more

4 mentions without citation

Sequence and structural features of carbohydrate binding in proteins and assessment of predictability using a neural network.
Malik et al. (2007)
3 more