› 1wac

X-ray diffraction
3Å resolution

Back-priming mode of Phi6 RNA-dependent RNA polymerase

Released:
DOI: 10.2210/pdb1wac/pdb
PDB entry 1wac coloured by chain, front view.
PDB entry 1wac coloured by chain, side view.
PDB entry 1wac coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
Back-priming mode of phi6 RNA-dependent RNA polymerase.
Laurila MRL, Salgado PS, Stuart DI, Grimes JM, Bamford DH
J Gen Virol 86 521-526 (2005)
PMID: 15659773

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 662 amino acids
Theoretical weight: 74.44 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 76.579Å b: 105.851Å c: 157.712Å
α: 90° β: 98.86° γ: 90°
R-values:
R R work R free
0.241 0.241 0.28
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure-function relationships among RNA-dependent RNA polymerases.
Ng et al. (2008)
4 more

1 mention without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)