› 1wbd

X-ray diffraction
2.4Å resolution

Crystal structure of E. coli DNA mismatch repair enzyme MutS, E38Q mutant, in complex with a G.T mismatch

Released:
DOI: 10.2210/pdb1wbd/pdb
PDB entry 1wbd coloured by chain, front view.
PDB entry 1wbd coloured by chain, side view.
PDB entry 1wbd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dual role of MutS glutamate 38 in DNA mismatch discrimination and in the authorization of repair.
Lebbink JH, Georgijevic D, Natrajan G, Fish A, Winterwerp HH, Sixma TK, de Wind N
EMBO J 25 409-19 (2006)
PMID: 16407973

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114763 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP *CP*AP*CP*CP*AP*GP*TP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.53 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*CP*TP*GP*GP*TP*GP*CP*TP*TP *GP*GP*CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 17 nucleotides
Theoretical weight: 5.23 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 89.62Å b: 92.042Å c: 260.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.259
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

DNA mismatch repair: molecular mechanism, cancer, and ageing.
Hsieh et al. (2008)
6 more

2 mentions without citation

Dual role of MutS glutamate 38 in DNA mismatch discrimination and in the authorization of repair.
Lebbink et al. (2006)
1 more