› 1wcl

Solution NMR

NMR structure of the carboxyterminal domains of Escherichia coli NusA

Released:
DOI: 10.2210/pdb1wcl/pdb
PDB entry 1wcl coloured by chain, ensemble of 19 models, front view.
PDB entry 1wcl coloured by chain, ensemble of 19 models, side view.
PDB entry 1wcl coloured by chain, ensemble of 19 models, top view.
Source organism: Escherichia coli
Primary publication:
The E. coli NusA carboxy-terminal domains are structurally similar and show specific RNAP- and lambdaN interaction.
Eisenmann A, Schwarz S, Prasch S, Schweimer K, Rösch P
Protein Sci 14 2018-29 (2005)
PMID: 15987884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFF6 (Residues: 351-426; Coverage: 15%)
Gene names: JW3138, b3169, nusA
Structure domains: 5' to 3' exonuclease, C-terminal subdomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: MANUAL
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 citation in other articles

NusA interaction with the α subunit of E. coli RNA polymerase is via the UP element site and releases autoinhibition.
Schweimer et al. (2011)
13 more

7 mentions without citation

The NusA N-terminal domain is necessary and sufficient for enhancement of transcriptional pausing via interaction with the RNA exit channel of RNA polymerase.
Ha et al. (2010)
6 more