› 1wd4

X-ray diffraction
2.07Å resolution

Crystal structure of arabinofuranosidase complexed with arabinose

Released:
DOI: 10.2210/pdb1wd4/pdb
PDB entry 1wd4 coloured by chain, front view.
PDB entry 1wd4 coloured by chain, side view.
PDB entry 1wd4 coloured by chain, top view.
Source organism: Aspergillus luchuensis
Primary publication:
Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose.
Miyanaga A, Koseki T, Matsuzawa H, Wakagi T, Shoun H, Fushinobu S
J Biol Chem 279 44907-14 (2004)
PMID: 15292273

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-L-arabinofuranosidase B Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 50.88 KDa
Source organism: Aspergillus luchuensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8NK89 (Residues: 19-499; Coverage: 100%)
Gene names: AKAW_08685, abfB
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand AHR 3 x AHR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 39.461Å b: 98.62Å c: 144.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.221
Expression system: Komagataella pastoris

Quick links

Citations

12 review citations

Carbohydrate binding modules: biochemical properties and novel applications.
Shoseyov et al. (2006)
11 more

4 mentions without citation

Understanding the folding-function tradeoff in proteins.
Gosavi S. (2013)
3 more