› 1wfx

X-ray diffraction
2.8Å resolution

Crystal Structure of APE0204 from Aeropyrum pernix

Released:
DOI: 10.2210/pdb1wfx/pdb
PDB entry 1wfx coloured by chain, front view.
PDB entry 1wfx coloured by chain, side view.
PDB entry 1wfx coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1.
Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S
J Mol Biol 348 295-305 (2005)
PMID: 15811369

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable RNA 2'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.4 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YFP5 (Residues: 35-220; Coverage: 85%)
Gene names: APE_0204.1, kptA
Sequence domains: RNA 2'-phosphotransferase, Tpt1 / KptA family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 5 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: R32
Unit cell:
a: 102.667Å b: 102.667Å c: 101.489Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.286
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Compartmentation of NAD+-dependent signalling.
Koch-Nolte et al. (2011)
3 more

10 mentions without citation

The natural history of ADP-ribosyltransferases and the ADP-ribosylation system.
Aravind et al. (2015)
9 more