› 1wky

X-ray diffraction
1.65Å resolution

Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module

Released:
DOI: 10.2210/pdb1wky/pdb
PDB entry 1wky coloured by chain, front view.
PDB entry 1wky coloured by chain, side view.
PDB entry 1wky coloured by chain, top view.
Source organism: Bacillus sp. JAMB-602
Primary publication:
Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate.
Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K
Acta Crystallogr D Biol Crystallogr 60 1490-2 (2004)
PMID: 15272186

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannanase Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 51.03 KDa
Source organism: Bacillus sp. JAMB-602
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q4W8M3 (Residues: 30-490; Coverage: 100%)
Gene name: amn5A
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand CA 1 x CA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 70.682Å b: 79.48Å c: 80.418Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.178 0.2
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

Microbial mannanases: an overview of production and applications.
Dhawan et al. (2007)
8 other articles

8 mentions without citation

Performance of ZDOCK and ZRANK in CAPRI rounds 13-19.
Hwang et al. (2010)
7 more