› 1wnt

X-ray diffraction
2.3Å resolution

Structure of the tetrameric form of Human L-Xylulose Reductase

Released:
DOI: 10.2210/pdb1wnt/pdb
PDB entry 1wnt coloured by chain, front view.
PDB entry 1wnt coloured by chain, side view.
PDB entry 1wnt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the tetrameric form of human L-Xylulose reductase: probing the inhibitor-binding site with molecular modeling and site-directed mutagenesis.
El-Kabbani O, Carbone V, Darmanin C, Ishikura S, Hara A
Proteins 60 424-32 (2005)
PMID: 15906319

Function and Biology Details

Reaction catalysed: 
Xylitol + NADP(+) = L-xylulose + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-xylulose reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 244 amino acids
Theoretical weight: 25.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7Z4W1 (Residues: 1-244; Coverage: 100%)
Gene names: DCXR, SDR20C1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 81.941Å b: 59.96Å c: 82.805Å
α: 90° β: 91.678° γ: 90°
R-values:
R R work R free
0.32 0.202 0.252
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Design and applications of biodegradable polyester tissue scaffolds based on endogenous monomers found in human metabolism.
Barrett et al. (2009)
2 more

2 mentions without citation

Diacetyl/l-Xylulose Reductase Mediates Chemical Redox Cycling in Lung Epithelial Cells.
Yang et al. (2017)
1 more