Function and Biology Details
Reaction catalysed:
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assemblies composition:
Assembly name:
Isoleucine--tRNA ligase (preferred)
PDBe Complex ID:
PDB-CPX-157524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoleucine--tRNA ligase
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 20.63 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: tRNA synthetases class I (I, L, M and V)
Structure domains: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
Length: 186 amino acids
Theoretical weight: 20.63 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P56690 (Residues: 200-385; Coverage: 18%)
Sequence domains: tRNA synthetases class I (I, L, M and V)
Structure domains: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SPRING-8 BEAMLINE BL26B1
Spacegroup:
P41212
Expression system: Escherichia coli BL21(DE3)
