› 1wp1

X-ray diffraction
2.56Å resolution

Crystal structure of the drug-discharge outer membrane protein, OprM

Released:
DOI: 10.2210/pdb1wp1/pdb
PDB entry 1wp1 coloured by chain, front view.
PDB entry 1wp1 coloured by chain, side view.
PDB entry 1wp1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the drug discharge outer membrane protein, OprM, of Pseudomonas aeruginosa: dual modes of membrane anchoring and occluded cavity end.
Akama H, Kanemaki M, Yoshimura M, Tsukihara T, Kashiwagi T, Yoneyama H, Narita S, Nakagawa A, Nakae T
J Biol Chem 279 52816-9 (2004)
PMID: 15507433

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein OprM Chains: A, B
Molecule details ›
Chains: A, B
Length: 474 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: R32
Unit cell:
a: 85.43Å b: 85.43Å c: 1,044.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.252 0.308
Expression system: Pseudomonas aeruginosa

Quick links

Citations

42 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
41 more

19 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
18 more