› 1wr3

Solution NMR

Solution structure of the first WW domain of Nedd4-2

Released:
DOI: 10.2210/pdb1wr3/pdb
PDB entry 1wr3 coloured by chain, ensemble of 20 models, front view.
PDB entry 1wr3 coloured by chain, ensemble of 20 models, side view.
PDB entry 1wr3 coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Entry authors: Kowalski K, Merkel AL, Booker GW

Function and Biology Details

Reactions catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase NEDD4-like Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 4.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8CFI0 (Residues: 221-254; Coverage: 3%)
Gene names: Kiaa0439, Nedd4b, Nedd4l
Sequence domains: WW domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli BL21

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