› 1wzz

X-ray diffraction
1.65Å resolution

Structure of endo-beta-1,4-glucanase CMCax from Acetobacter xylinum

Released:
DOI: 10.2210/pdb1wzz/pdb
PDB entry 1wzz coloured by chain, front view.
PDB entry 1wzz coloured by chain, side view.
PDB entry 1wzz coloured by chain, top view.
Source organism: Komagataeibacter xylinus
Primary publication:
Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis.
Yasutake Y, Kawano S, Tajima K, Yao M, Satoh Y, Munekata M, Tanaka I
Proteins 64 1069-77 (2006)
PMID: 16804941

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.48 KDa
Source organism: Komagataeibacter xylinus
Expression system: Escherichia coli
UniProt:
  • Canonical: P37696 (Residues: 21-342; Coverage: 100%)
Gene name: cmcAX
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P61
Unit cell:
a: 88.878Å b: 88.878Å c: 93.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.176 0.195
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A molecular description of cellulose biosynthesis.
McNamara et al. (2015)
2 more

8 mentions without citation

Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions.
Römling et al. (2015)
7 more