› 1x18

Electron Microscopy
13.5Å resolution

Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT

Released:
DOI: 10.2210/pdb1x18/pdb
PDB entry 1x18 coloured by chain, front view.
PDB entry 1x18 coloured by chain, side view.
PDB entry 1x18 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly.
Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, Agrawal RK
Mol Cell 18 319-29 (2005)
PMID: 15866174

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-148022 (preferred)
Entry contents:
5 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (9 distinct):
Small ribosomal subunit protein uS2 Chain: E
Molecule details ›
Chain: E
Length: 231 amino acids
Theoretical weight: 26.67 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80371 (Residues: 7-240; Coverage: 90%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS7 Chain: F
Molecule details ›
Chain: F
Length: 154 amino acids
Theoretical weight: 17.82 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P17291 (Residues: 2-156; Coverage: 99%)
Gene names: TTHA1696, rps7, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS11 Chain: G
Molecule details ›
Chain: G
Length: 119 amino acids
Theoretical weight: 12.61 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80376 (Residues: 11-129; Coverage: 92%)
Gene names: TTHA1666, rps11, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein bS18 Chain: H
Molecule details ›
Chain: H
Length: 73 amino acids
Theoretical weight: 8.48 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLQ0 (Residues: 16-88; Coverage: 83%)
Gene names: TTHA0243, rpsR
Sequence domains: Ribosomal protein S18
GTP-binding protein era Chain: X
Molecule details ›
Chain: X
Length: 292 amino acids
Theoretical weight: 32.86 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
5'-R(P*CP*GP*AP*UP*GP*GP*CP*GP*AP*AP*G)-3' Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.59 KDa
RNA (31-MER) Chain: B
Molecule details ›
Chain: B
Length: 31 nucleotides
Theoretical weight: 9.97 KDa
5'-R(P*UP*UP*CP*CP*CP*GP*GP*GP*CP*CP*UP*GP*GP*GP*GP*CP*CP*CP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.38 KDa
5'-R(P*UP*GP*UP*UP*GP*GP*GP*UP*UP*AP*AP*GP*UP*CP*CP*CP*GP*CP*AP*AP*CP*GP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.73 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.5Å
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Ribosome biogenesis and the translation process in Escherichia coli.
Kaczanowska et al. (2007)
19 more

2 mentions without citation

Structural insights into methyltransferase KsgA function in 30S ribosomal subunit biogenesis.
Boehringer et al. (2012)
1 more