› 1x6z

X-ray diffraction
0.78Å resolution

Structure 1: cryocooled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.78A resolution

Released:
DOI: 10.2210/pdb1x6z/pdb
PDB entry 1x6z coloured by chain, front view.
PDB entry 1x6z coloured by chain, side view.
PDB entry 1x6z coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Pros and cons of cryocrystallography: should we also collect a room-temperature data set?
Dunlop KV, Irvin RT, Hazes B
Acta Crystallogr D Biol Crystallogr 61 80-7 (2005)
PMID: 15608379

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fimbrial protein Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 12.7 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02973 (Residues: 35-150; Coverage: 77%)
Gene names: fimA, pilA
Sequence domains: Pilin (bacterial filament)
Structure domains: Glycoprotein, Type 4 Pilin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 37.244Å b: 37.244Å c: 147.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.154
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Type IV pilin proteins: versatile molecular modules.
Giltner et al. (2012)
3 more

6 mentions without citation

Protonate3D: assignment of ionization states and hydrogen coordinates to macromolecular structures.
Labute P. (2009)
5 more