› 1x8j

X-ray diffraction
2.35Å resolution

Crystal structure of retinol dehydratase in complex with androsterone and inactive cofactor PAP

Released:
DOI: 10.2210/pdb1x8j/pdb
PDB entry 1x8j coloured by chain, front view.
PDB entry 1x8j coloured by chain, side view.
PDB entry 1x8j coloured by chain, top view.
Source organism: Spodoptera frugiperda
Primary publication:
The structures of the unique sulfotransferase retinol dehydratase with product and inhibitors provide insight into enzyme mechanism and inhibition.
Pakhomova S, Buck J, Newcomer ME
Protein Sci 14 176-82 (2005)
PMID: 15608121

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 41.52 KDa
Source organism: Spodoptera frugiperda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26490 (Residues: 1-351; Coverage: 100%)
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand A3P 2 x A3P
Ligand AOI 2 x AOI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 81.767Å b: 66.762Å c: 84.503Å
α: 90° β: 111.17° γ: 90°
R-values:
R R work R free
0.22 0.22 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Quantum chemistry rules retinoid biology.
Hammerling et al. (2023)
6 other articles

1 mention without citation

The structures of the unique sulfotransferase retinol dehydratase with product and inhibitors provide insight into enzyme mechanism and inhibition.
Pakhomova et al. (2005)