› 1xbt

X-ray diffraction
2.4Å resolution

Crystal Structure of Human Thymidine Kinase 1

Released:
DOI: 10.2210/pdb1xbt/pdb
PDB entry 1xbt coloured by chain, front view.
PDB entry 1xbt coloured by chain, side view.
PDB entry 1xbt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of thymidine kinase 1 of human and mycoplasmic origin.
Welin M, Kosinska U, Mikkelsen NE, Carnrot C, Zhu C, Wang L, Eriksson S, Munch-Petersen B, Eklund H
Proc Natl Acad Sci U S A 101 17970-5 (2004)
PMID: 15611477

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase, cytosolic Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 193 amino acids
Theoretical weight: 21.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04183 (Residues: 1-193; Coverage: 83%)
Gene name: TK1
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 8 x MG
Ligand TTP 8 x TTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 157.672Å b: 123.177Å c: 116Å
α: 90° β: 130.2° γ: 90°
R-values:
R R work R free
0.203 0.201 0.232
Expression system: Escherichia coli

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Citations

9 review citations

Single amino acid chelates (SAAC): a strategy for the design of technetium and rhenium radiopharmaceuticals.
Bartholomä et al. (2009)
8 more

7 mentions without citation

Structures of thymidine kinase 1 of human and mycoplasmic origin.
Welin et al. (2004)
6 more