› 1xez

X-ray diffraction
2.3Å resolution

Crystal Structure Of The Vibrio Cholerae Cytolysin (HlyA) Pro-Toxin With Octylglucoside Bound

Released:
DOI: 10.2210/pdb1xez/pdb
PDB entry 1xez coloured by chain, front view.
PDB entry 1xez coloured by chain, side view.
PDB entry 1xez coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of the Vibrio cholerae cytolysin (VCC) pro-toxin and its assembly into a heptameric transmembrane pore.
Olson R, Gouaux E
J Mol Biol 350 997-1016 (2005)
PMID: 15978620

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemolysin Chain: A
Molecule details ›
Chain: A
Length: 721 amino acids
Theoretical weight: 79.65 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P09545 (Residues: 25-741; Coverage: 100%)
Gene names: VC_A0219, hlyA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 81.211Å b: 97.321Å c: 135.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.207 0.246
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
10 more

21 mentions without citation

Signaling beyond Punching Holes: Modulation of Cellular Responses by Vibrio cholerae Cytolysin.
Khilwani et al. (2015)
20 more