› 1xf6

X-ray diffraction
1.1Å resolution

High resolution crystal structure of phycoerythrin 545 from the marine cryptophyte rhodomonas CS24

Released:
DOI: 10.2210/pdb1xf6/pdb
PDB entry 1xf6 coloured by chain, front view.
PDB entry 1xf6 coloured by chain, side view.
PDB entry 1xf6 coloured by chain, top view.
Source organism: Rhodomonas sp. CS24
Primary publication:
Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy.
Doust AB, Marai CN, Harrop SJ, Wilk KE, Curmi PM, Scholes GD
J Mol Biol 344 135-53 (2004)
PMID: 15504407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150949 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Phycoerythrin alpha-3 chain, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.14 KDa
Source organism: Rhodomonas sp. CS24
UniProt:
  • Canonical: Q00433 (Residues: 53-128; Coverage: 59%)
Gene name: cpeA3
Sequence domains: Phycoerythrin, alpha/beta chain
Structure domains: Phycoerythrin alpha chain
Phycoerythrin alpha-2 chain, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.03 KDa
Source organism: Rhodomonas sp. CS24
UniProt:
  • Canonical: P30943 (Residues: 38-104; Coverage: 64%)
Gene name: cpeA2
Sequence domains: Phycoerythrin, alpha/beta chain
Structure domains: Phycoerythrin alpha chain
B-phycoerythrin beta chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 177 amino acids
Theoretical weight: 18.54 KDa
Source organism: Rhodomonas sp. CS24
UniProt:
  • Canonical: P27198 (Residues: 1-177; Coverage: 100%)
Gene name: cpeB
Sequence domains: Phycobilisome protein
Structure domains: Phycocyanins

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand DBV 2 x DBV
Ligand CL 1 x CL
Ligand PEB 6 x PEB
2 modified residues:
Ligand LYZ 1 x LYZ
Ligand MEN 2 x MEN

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 62.98Å b: 82.555Å c: 89.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.097 0.096 0.116

Quick links

Citations

4 review citations

Biliprotein maturation: the chromophore attachment.
Scheer et al. (2008)
3 more