› 1xhk

X-ray diffraction
1.9Å resolution

Crystal structure of M. jannaschii Lon proteolytic domain

Released:
DOI: 10.2210/pdb1xhk/pdb
PDB entry 1xhk coloured by chain, front view.
PDB entry 1xhk coloured by chain, side view.
PDB entry 1xhk coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
The active site of a lon protease from Methanococcus jannaschii distinctly differs from the canonical catalytic Dyad of Lon proteases.
Im YJ, Na Y, Kang GB, Rho SH, Kim MK, Lee JH, Chung CH, Eom SH
J Biol Chem 279 53451-7 (2004)
PMID: 15456757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Archaeal Lon protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 20.1 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58812 (Residues: 456-640; Coverage: 29%)
Gene name: MJ1417
Sequence domains: Lon protease (S16) C-terminal proteolytic domain
Structure domains: Ribosomal Protein S5; domain 2

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand MES 1 x MES
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P42212
Unit cell:
a: 89.846Å b: 89.846Å c: 100.609Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Slicing a protease: structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains.
Rotanova et al. (2006)
8 more

6 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
5 more