› 1xil

X-ray diffraction
1.53Å resolution

HYDROGEN BONDING IN HUMAN MANGANESE SUPEROXIDE DISMUTASE CONTAINING 3-FLUOROTYROSINE

Released:
DOI: 10.2210/pdb1xil/pdb
PDB entry 1xil coloured by chain, front view.
PDB entry 1xil coloured by chain, side view.
PDB entry 1xil coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hydrogen bonding in human manganese superoxide dismutase containing 3-fluorotyrosine.
Ayala I, Perry JJ, Szczepanski J, Tainer JA, Vala MT, Nick HS, Silverman DN
Biophys J 89 4171-9 (2005)
PMID: 16150974

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04179 (Residues: 25-222; Coverage: 89%)
Gene name: SOD2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand YOF 16 x YOF

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 73.696Å b: 75.309Å c: 67.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 not available not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The structural biochemistry of the superoxide dismutases.
Perry et al. (2010)
2 more

2 mentions without citation

The structure-function relationships and physiological roles of MnSOD mutants.
Bonetta Valentino R. (2022)
1 more