› 1xip

X-ray diffraction
2.5Å resolution

Crystal Structure of the N-terminal Domain of Nup159

Released:
DOI: 10.2210/pdb1xip/pdb
PDB entry 1xip coloured by chain, front view.
PDB entry 1xip coloured by chain, side view.
PDB entry 1xip coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.
Weirich CS, Erzberger JP, Berger JM, Weis K
Mol Cell 16 749-60 (2004)
PMID: 15574330

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoporin NUP159 Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.18 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40477 (Residues: 2-387; Coverage: 26%)
Gene names: NUP158, NUP159, RAT7, YIL115C
Sequence domains: Nucleoporin or Nuclear pore complex subunit NUP214=Nup159
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 60.359Å b: 71.516Å c: 90.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
30 more

4 mentions without citation

A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export.
Montpetit et al. (2011)
3 more